Medical Director

Alissandro "Andy" Castillo, MD, MBA

Dr. Andy Castillo is a board-certified physician, and the owner and Medical Director of De Novo Aesthetics and M.D. Laser Studio. His education includes a BS from the University of Maryland, an MD from Wake Forest University, and an MBA from Duke University Fuqua School of Business. While serving in the United States Army as a flight surgeon he also successfully completed a plastic and reconstructive surgery research fellowship at prestigious Johns Hopkins. WIth his unique combination of procedural protocol and technique, Dr. Castillo has trained countless other nurses and physicians how to administer Botox. He has personally treated or directly supervised nearly 20,000 Botox treatments for wrinkle revision, hyperhidrosis, migraines, and TMJ,

Medical Director

Rebecca Appleton Castillo, MD, MS

Dr. Appleton, the wife of Dr. Castillo, is a board-certified physician and Co-Founder and Co-Medical Director for De Novo Aesthetics. She earned a BS from Duke University and a MS from University of Maryland. She then completed her Doctor of Medicine degree at the University of Maryland Medical School. Dr. Appleton and Dr. Castillo have raised 6 children and owned and operated 5 successful medical practices. In addition to overseeing operations for De Novo, she and Dr. Castillo offer CME courses to fellow physicians and nurses. Dr. Appleton directly reviews the medical history of every potential Botox Express patient.

Certified Botox Injection Specialist ~ Lake Norman

Kendra Jackson, RN, BSN

Kendra is one of those rare finds: a native North Carolinian! She earned her BSN at University of North Carolina - Charlotte, graduating in December of 2012. She is currently pursuing her Master of Science in Nursing Family Nurse Practitioner accreditation at the University of South Carolina - Columbia. In addition to being our De Novo Aesthetics Nurse Coordinator, Kendra is a new mom!

Certified Botox Injection Specialist ~ Lake Norman

Cara Wicker, RN, BSN

Cara has been a nurse since 2010 in addition to being a mother of three. She is a Certified Botox Injection Specialist and also serves as HIPAA Coordinator for De Novo, helping to ensure that all processes are in place to protect patient privacy. Cara manages our Instagram account: be sure to follow us @denovoaestheticss and follow Cara @aestheticsnursecara!

Certified Botox Injection Specialist ~ Lake Norman

Allison Robinson, RN, BSN

Allison earned her BSN from Queens College in 1996. Born and raised Charlotte, Allison and her three kids enjoy watching and playing sports and consider themselves true blue NC Tarheels fans! She became a Certified Botox Injection Specialist for DeNovo in 2017 joining our Lake Norman team.

Certified Botox Injection Specialist ~ Charlotte

Shanti Coates, RN, BSN

Born and raised in Florida, Shanti earned her BSN at University of Florida. She and her husband moved to NC to take advantage of its proximity to both the ocean and the mountains. Shanti enjoys the outdoors and especially loves yoga. Her hobbies include hiking, painting, traveling, and playing with her plump pug, Tank! You can find Shanti at our Charlotte location, and online @aestheticsnurseshanti.

Certified Botox Injection Specialist ~ Charlotte

Carrie Barnhart, RN, BSN

After years of serving as an RN in Kansas City, MO, raising three children, and earning her BSN, Carrie was recruited to help start the Bone Marrow Transplant program at Levine's Children's Hospital in Charlotte. She is currently a clinical analyst for a transplant software company and a Certified Botox Injection Specialist for our Charlotte location!

Operations Administrator

Sarah Stephens

Sarah oversees the administration of our paperless, tech-enabled medical practice. She ensures that the doctors and nurses have all the necessary equipment and products, and that all data exchanged is private and encrypted. She also helps coordinate schedules and appointments.

MD = Doctor of Medicine
MBA = Masters of Business Administration
MS = Master of Science
RN = Registered Nurse
BSN = Bachelor of Science in Nursing

Marketing Director

Rita Aldridge

Rita handles all advertising and promotions for De Novo Aesthetics. She is responsible for all creative content, including writing, design, photos, and videos, in addition to media exposure. If you are interested in co-promotions with De Novo or would like additional information, contact Rita at

For full-service medical cosmetic services, check out M.D. Laser Studio.

M.D.Laser Studio

Mission Statement

We will bring clinical knowledge, skills, and experience in medical cosmetic treatments to enhance and maintain the natural youthful appearance of our clients in the comfort and security of their home or one of our convenient locations.